2024 / 01

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DOI: 10.24075/brsmu.2024.007

ORIGINAL RESEARCH

Information capacity of the NF-κB and AP-1 signaling activation sensors in in vitro assessment of dermatotoxic effects

Tolstova TV, Pureczcky VK, Kozhin PM, Luzgina NG, Rusanov AL
About authors

Institute of Biomedical Chemistry, Moscow, Russia

Correspondence should be addressed: Tatiana V. Tolstova
Pogodinskaya, 10, s. 8, Moscow, 119121, Russia; ur.ksm.cmbi@avotslot

About paper

Funding: the study was performed as part of the Programme for Fundamental Scientific Research in the Russian Federation for the long-term period (2021–2030) (№ 122022800481-0).

Author contribution: Tolstova TV — experimental procedure, data acquisition, analysis, and interpretation, manuscript writing, illustrations; Pureczcky VK — experimental procedure, illustrations; Kozhin PM — literature review, manuscript writing, illustrations; Luzgina NG — study concept and design, manuscript writing; Rusanov AL — study planning, manuscript writing, overall guidance for the study.

Received: 2023-12-11 Accepted: 2024-02-16 Published online: 2024-02-27
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Fig. 1. Fluorescence intensity of transduced cells: control cells (А, C) and transduced cells showing high fluorescence intensity (B, D) in response to stimulation with 20 ng/mL TNFα for HaCaT/NF-κB (gate Р1) and 10 µМ Cd(NO3)2 for HaCaT/AP-1 (gate Р2) throughout 24 h
Fig. 2. Changes in the HaCaT/NF-κB cells’ relative fluorescence intensity under exposure to TNFα (А, B) and LPS (C, D). Fluorometry results (А, C) and fluorescence microscopy (B, D). * — significant differences from the previously reported concentration, p < 0.05.
Fig. 3. Assessing activation of the genes encoding the NF-κB transcription factor subunits. Relative expression of RelA and NFKB1 mRNA under exposure to TNFα (А, B) and LPS (C, D). * — significant differences from the control group, p < 0.05
Fig. 4. Assessing activation of the gene encoding the AP-1 transcription factor subunit. Relative expression of the C-JUN mRNA under exposure to Cd(NO3)2 (А) and UVA (B). * — significant differences from the control group, p < 0.05
Table 1. Sequences of PCR primers
Table 2. Changes in the HaCaT/AP-1 cell fluorescence associated with exposure to Cd(NO3)2
Note: * — significant differences from the control group, p < 0.05